Modulation of Breast Cancer Cell Apoptosis and Macrophage Polarization by Mistletoe Lectin in 2D and 3D Models

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dc.contributor.authorHong, Chang-Eui
dc.contributor.authorLyu, Su-Yun
dc.date.accessioned2024-09-13T18:27:08Z
dc.date.available2024-09-13T18:27:08Z
dc.date.issued2024-08
dc.description.abstractKorean mistletoe (Viscum album L. var. coloratum) is renowned for its medicinal properties, including anti-cancer and immunoadjuvant effects. This study aimed to elucidate the mechanisms by which Korean mistletoe lectin (V. album L. var. coloratum agglutinin; VCA) modulates breast cancer cell apoptosis and macrophage polarization. The specific objectives were to (1) investigate the direct effects of VCA on MCF-7 breast cancer cells and THP-1-derived M1/M2 macrophages; (2) analyze the impact of VCA on the paracrine interactions between these cell types; and (3) compare the efficacy of VCA in 2D vs. 3D co-culture models to bridge the gap between in vitro and in vivo studies. We employed both 2D and 3D models, co-culturing human M1/M2 macrophages with human MCF-7 breast cancer cells in a Transwell system. Our research demonstrated that M1 and M2 macrophages significantly influenced the immune and apoptotic responses of breast cancer cells when exposed to VCA. M1 macrophages exhibited cytotoxic characteristics and enhanced VCA-induced apoptosis in both 2D and 3D co-culture models. Conversely, M2 macrophages initially displayed a protective effect by reducing apoptosis in breast cancer cells, but this protective effect was reversed upon exposure to VCA. Furthermore, our findings illustrate VCA’s ability to modulate M1 and M2 polarization in breast cancer cells. Finally, the use of magnetic 3D cell cultures suggests their potential to yield results comparable to conventional 2D cultures, bridging the gap between in vitro and in vivo studies.
dc.identifier.citationHong, C.-E., & Lyu, S.-Y. (2024). Modulation of Breast Cancer Cell Apoptosis and Macrophage Polarization by Mistletoe Lectin in 2D and 3D Models. International Journal of Molecular Sciences, 25(15), Article 15. https://doi.org/10.3390/ijms25158459
dc.identifier.doihttps://doi.org/10.3390/ijms25158459
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/20.500.14430/691
dc.relation.urldc.descr.uri
dc.subjectMCF-7
dc.subject3D cell culture
dc.subjectM1 macrophage
dc.subjectM2 macrophage
dc.subjectMistletoe lectin
dc.subjectViscum album L
dc.subjectBreast cancer
dc.subject.meshViscum album
dc.titleModulation of Breast Cancer Cell Apoptosis and Macrophage Polarization by Mistletoe Lectin in 2D and 3D Models
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleIntl J Molecular Sciences
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